cells cell primary prostate atcc pcs 440 010 Search Results


prostate epithelial cell growth kit  (ATCC)
94
ATCC prostate epithelial cell growth kit
Prostate Epithelial Cell Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cells+cell+primary+prostate+atcc+pcs+440+010/Prostate+Epithelial+Cell+Growth+Kit/pmc07416765-477-13-18
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prec basal medium  (ATCC)
99
ATCC prec basal medium
Fig. 5 LEADR regulates basal–luminal differentiation. a Bright-field images showing morphological changes occurring in RWPE-1 (left) and <t>PrEC</t> (right) cells (day 3) upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4c. b qRT-PCR showing changes in basal and luminal cytokeratins in RWPE-1 or PrEC cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Mean + s.d. plotted. c Western blots showing changes in basal/luminal cytokeratins in RWPE-1 (left) or PrEC (right) cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Vinculin used as loading control. d Immunofluorescence showing cytoplasmic re-localization of p63 (green) upon LEADR silencing in RWPE-1 cells by siLEADR (top) or gapLEADR (bottom). Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images are reported in Supplementary Fig. 4d. e Western blots showing changes in Androgen Receptor (AR) expression in PrEC cells upon LEADR silencing by siLEADR (left) or gapLEADR (right). GAPDH used as loading control. f Immunofluorescence showing AR (red) expression in PrEC cells upon LEADR silencing by siLEADR, in the presence or absence of simultaneous DHT stimulation. Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4g. g ELISA-based quantification of PSA in the conditioned media of PrEC cells silenced for LEADR expression by siLEADR (±DHT). Mean + s.d. (n = 2) plotted. h Heatmap (bottom) of the normalized enrichment score (NES) for luminal and basal custom gene sets (obtained from gene- expression data of frankly basal and <t>luminal</t> <t>prostate</t> cells from the indicated datasets) in RWPE-1 cells upon knockdown of LEADR and/or miR-205 (the latter abrogated using an antisense LNA-modified oligomer, LNA205). Bar plot of differential luminal/basal NES is reported (top). Mean + s.d. (n = 4) plotted. i Plot showing propensity of wild type (wt), LEADR-overexpressing and CRISPRed RWPE-1 cells to differentiate toward luminal phenotype (measured by qRT-PCR and expressed as KRT18/KRT5 ratio) upon culturing in media with increasing differentiative potential (n = 3). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 (Student’s t test). Source data are provided as a Source Data file, together with n of all experiments
Prec Basal Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cells+cell+primary+prostate+atcc+pcs+440+010/Prostate+Epithelial+Cell+Basal+Medium/pm30659180-224-10-13
Average 99 stars, based on 1 article reviews
prec basal medium - by Bioz Stars, 2026-09
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basal mediums  (ATCC)
92
ATCC basal mediums
Fig. 5 LEADR regulates basal–luminal differentiation. a Bright-field images showing morphological changes occurring in RWPE-1 (left) and <t>PrEC</t> (right) cells (day 3) upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4c. b qRT-PCR showing changes in basal and luminal cytokeratins in RWPE-1 or PrEC cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Mean + s.d. plotted. c Western blots showing changes in basal/luminal cytokeratins in RWPE-1 (left) or PrEC (right) cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Vinculin used as loading control. d Immunofluorescence showing cytoplasmic re-localization of p63 (green) upon LEADR silencing in RWPE-1 cells by siLEADR (top) or gapLEADR (bottom). Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images are reported in Supplementary Fig. 4d. e Western blots showing changes in Androgen Receptor (AR) expression in PrEC cells upon LEADR silencing by siLEADR (left) or gapLEADR (right). GAPDH used as loading control. f Immunofluorescence showing AR (red) expression in PrEC cells upon LEADR silencing by siLEADR, in the presence or absence of simultaneous DHT stimulation. Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4g. g ELISA-based quantification of PSA in the conditioned media of PrEC cells silenced for LEADR expression by siLEADR (±DHT). Mean + s.d. (n = 2) plotted. h Heatmap (bottom) of the normalized enrichment score (NES) for luminal and basal custom gene sets (obtained from gene- expression data of frankly basal and <t>luminal</t> <t>prostate</t> cells from the indicated datasets) in RWPE-1 cells upon knockdown of LEADR and/or miR-205 (the latter abrogated using an antisense LNA-modified oligomer, LNA205). Bar plot of differential luminal/basal NES is reported (top). Mean + s.d. (n = 4) plotted. i Plot showing propensity of wild type (wt), LEADR-overexpressing and CRISPRed RWPE-1 cells to differentiate toward luminal phenotype (measured by qRT-PCR and expressed as KRT18/KRT5 ratio) upon culturing in media with increasing differentiative potential (n = 3). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 (Student’s t test). Source data are provided as a Source Data file, together with n of all experiments
Basal Mediums, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cells+cell+primary+prostate+atcc+pcs+440+010/Prostate+Epithelial+Cell+Basal+Medium/pmc06801752-113-27-29
Average 92 stars, based on 1 article reviews
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Image Search Results


Fig. 5 LEADR regulates basal–luminal differentiation. a Bright-field images showing morphological changes occurring in RWPE-1 (left) and PrEC (right) cells (day 3) upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4c. b qRT-PCR showing changes in basal and luminal cytokeratins in RWPE-1 or PrEC cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Mean + s.d. plotted. c Western blots showing changes in basal/luminal cytokeratins in RWPE-1 (left) or PrEC (right) cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Vinculin used as loading control. d Immunofluorescence showing cytoplasmic re-localization of p63 (green) upon LEADR silencing in RWPE-1 cells by siLEADR (top) or gapLEADR (bottom). Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images are reported in Supplementary Fig. 4d. e Western blots showing changes in Androgen Receptor (AR) expression in PrEC cells upon LEADR silencing by siLEADR (left) or gapLEADR (right). GAPDH used as loading control. f Immunofluorescence showing AR (red) expression in PrEC cells upon LEADR silencing by siLEADR, in the presence or absence of simultaneous DHT stimulation. Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4g. g ELISA-based quantification of PSA in the conditioned media of PrEC cells silenced for LEADR expression by siLEADR (±DHT). Mean + s.d. (n = 2) plotted. h Heatmap (bottom) of the normalized enrichment score (NES) for luminal and basal custom gene sets (obtained from gene- expression data of frankly basal and luminal prostate cells from the indicated datasets) in RWPE-1 cells upon knockdown of LEADR and/or miR-205 (the latter abrogated using an antisense LNA-modified oligomer, LNA205). Bar plot of differential luminal/basal NES is reported (top). Mean + s.d. (n = 4) plotted. i Plot showing propensity of wild type (wt), LEADR-overexpressing and CRISPRed RWPE-1 cells to differentiate toward luminal phenotype (measured by qRT-PCR and expressed as KRT18/KRT5 ratio) upon culturing in media with increasing differentiative potential (n = 3). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 (Student’s t test). Source data are provided as a Source Data file, together with n of all experiments

Journal: Nature communications

Article Title: LEADeR role of miR-205 host gene as long noncoding RNA in prostate basal cell differentiation.

doi: 10.1038/s41467-018-08153-2

Figure Lengend Snippet: Fig. 5 LEADR regulates basal–luminal differentiation. a Bright-field images showing morphological changes occurring in RWPE-1 (left) and PrEC (right) cells (day 3) upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4c. b qRT-PCR showing changes in basal and luminal cytokeratins in RWPE-1 or PrEC cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Mean + s.d. plotted. c Western blots showing changes in basal/luminal cytokeratins in RWPE-1 (left) or PrEC (right) cells upon LEADR silencing by siLEADR (top) or gapLEADR (bottom). Vinculin used as loading control. d Immunofluorescence showing cytoplasmic re-localization of p63 (green) upon LEADR silencing in RWPE-1 cells by siLEADR (top) or gapLEADR (bottom). Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images are reported in Supplementary Fig. 4d. e Western blots showing changes in Androgen Receptor (AR) expression in PrEC cells upon LEADR silencing by siLEADR (left) or gapLEADR (right). GAPDH used as loading control. f Immunofluorescence showing AR (red) expression in PrEC cells upon LEADR silencing by siLEADR, in the presence or absence of simultaneous DHT stimulation. Nuclei counterstained with DAPI (blue). Scale bar, 50 µm. Full-size images reported in Supplementary Fig. 4g. g ELISA-based quantification of PSA in the conditioned media of PrEC cells silenced for LEADR expression by siLEADR (±DHT). Mean + s.d. (n = 2) plotted. h Heatmap (bottom) of the normalized enrichment score (NES) for luminal and basal custom gene sets (obtained from gene- expression data of frankly basal and luminal prostate cells from the indicated datasets) in RWPE-1 cells upon knockdown of LEADR and/or miR-205 (the latter abrogated using an antisense LNA-modified oligomer, LNA205). Bar plot of differential luminal/basal NES is reported (top). Mean + s.d. (n = 4) plotted. i Plot showing propensity of wild type (wt), LEADR-overexpressing and CRISPRed RWPE-1 cells to differentiate toward luminal phenotype (measured by qRT-PCR and expressed as KRT18/KRT5 ratio) upon culturing in media with increasing differentiative potential (n = 3). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 (Student’s t test). Source data are provided as a Source Data file, together with n of all experiments

Article Snippet: The normal primary prostate epithelial cells (PrEC) were grown in PrEC basal medium (ATCC; PCS-440-030) supplemented with PrEC growth kit (ATCC; PCS-440-040).

Techniques: Quantitative RT-PCR, Western Blot, Control, Expressing, Enzyme-linked Immunosorbent Assay, Gene Expression, Knockdown